The antisense strand, one of two DNA strands that function as templates, is always used to start transcription of a certain gene. The RNA product is complementary to the template DNA strand and almost similar to the non-template DNA strand, also known as the sense strand. The rule of using antisense technology is that antisense macromolecule sequence base pairs with its complementary sense RNA strand and prevents it from being translated into a protein (Stuti Gupta et al., 2011). Using antisense RNAs complementary for the mRNA changes the organic phenomenon in crops. Wherever a gene exists on a DNA molecule, one strand is the coding strand (or sense strand ), and the other is the noncoding strand (also called the antisense strand, [3] anticoding strand, template strand or transcribed strand ). Strands in transcription bubble Antisense transcripts. Antisense transcripts are stretches of non coding mRNA that are complementary to the coding sequence. Genome wide studies have shown that RNA antisense transcripts occur commonly within nature. They are generally believed to increase the coding potential of the genetic code and add an overall layer of complexity to gene A DNA antisense strand contains the following nucleotide base sequence: ATC CAA GAC TGG From this, what is the nucleotide sequence of the mRNA strand that is transcribed? Select one: a. Antisense oligonucleotides have one strand that can block RNA by a steric mechanism or form a DNA-mRNA hybrid that recruits RNase H . Because of these differences the antisense experience will not Antisense therapy means the selective, sequence-specific inhibition of gene expression by single-stranded DNA oligonucleotides. In contrast, RNA interference (RNAi) is triggered by double-stranded RNA (dsRNA) and causes sequence-specific mRNA degradation of single-stranded target RNAs in response to dsRNA. The mediators of mRNA degradation are Antisense strand serves as the template for the transcription, and contains complementary nucleotide sequence to the transcribed mRNA. Therefore, antisense strand is responsible for translating proteins. The main difference between sense and antisense strand is that sense strand is incapable of being transcribed into mRNA whereas antisense Abstract. Antisense oligonucleotides (ASOs) are short oligonucleotides that can modify gene expression and mRNA splicing in the nervous system. The FDA has approved ASOs for treatment of ten genetic disorders, with many applications currently in the pipeline. We describe the molecular mechanisms of ASO treatment for four neurodevelopmental and neuromuscular disorders. The ASO nusinersen is a One method of gene suppression is through antisense oligonucleotides (ASOs). ASOs are small single-stranded chemically modified DNA molecules that use Watson Crick base pairing to bind RNA and catalyze downstream events . Gene suppressing ASOs form DNA/RNA heteroduplexes when bound, recruiting RNase H to degrade the targeted transcript. sQycls.